DNA sequence differences of ampD mutants of Citrobacter freundii
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چکیده
منابع مشابه
NMR structure of Citrobacter freundii AmpD, comparison with bacteriophage T7 lysozyme and homology with PGRP domains.
AmpD is a bacterial amidase involved in the recycling of cell-wall fragments in Gram-negative bacteria. Inactivation of AmpD leads to derepression of beta-lactamase expression, presenting a major pathway for the acquisition of constitutive antibiotic resistance. Here, we report the NMR structure of AmpD from Citrobacter freundii (PDB accession code 1J3G). A deep substrate-binding pocket explain...
متن کاملMutants of Citrobacter freundii that transport and utilize melibiose.
We have isolated mutants of Citrobacter freundii that can grow on melibiose. Inducible alpha-galactosidase activity and melibiose transport activity were detected in the mutant cells but not in the wild-type cells. We detected a DNA region which hybridized with melB (the gene for the melibiose transporter) DNA of Escherichia coli in the chromosomal DNA of wild-type C. freundii. Protons, but not...
متن کاملMutational analysis of the catalytic centre of the Citrobacter freundii AmpD N-acetylmuramyl-L-alanine amidase.
Citrobacter freundii AmpD is an intracellular 1,6-anhydro-N-acetylmuramyl-L-alanine amidase involved in both peptidoglycan recycling and beta-lactamase induction. AmpD exhibits a strict specificity for 1,6-anhydromuropeptides and requires zinc for enzymic activity. The AmpD three-dimensional structure exhibits a fold similar to that of another Zn2+ N-acetylmuramyl-L-alanine amidase, the T7 lyso...
متن کاملComplete Genome Sequence of Citrobacter freundii Myophage Merlin
Bacteriophage Merlin is a T4-like myophage which infects Citrobacter freundii, a member of the Enterobacteriaceae family. C. freundii is an opportunistic pathogen that is a common cause of nosocomial infections. This report announces the complete genome of myophage Merlin and describes its features.
متن کاملComplete Genome Sequence of Citrobacter freundii Myophage Moogle
Citrobacter freundii is an opportunistic pathogen that has been linked to nosocomial infections, such as brain abscesses and pneumonia. Further study on phages infecting C. freundii may provide therapeutics for these infections. Here, we announce the complete genome sequence of the FelixO1-like myophage Moogle and describe its features.
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 1995
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.39.11.2494